- Title
- The potential of Elephantorrhiza elephantina as an anthelmintic of goats in the Eastern Cape province, South Africa
- Creator
- Mazhangara, Irene Rumbidza
- Subject
- Bioactive compounds -- South Africa -- Eastern Cape Medicinal plants -- South Africa -- Eastern Cape
- Date
- 2017
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10353/15068
- Identifier
- vital:40160
- Description
- The main objective of the study was to investigate the potential of Elephantorrhiza elephantina in the control of gastro-intestinal worms in goats in the Eastern Cape Province, South Africa. The ethanolic, methanolic and aqueous phytochemical composition of E. elephantina root bark extracts were determined. The phytochemical analysis revealed a high concentration of alkaloids, condensed tannins, flavonoids, phenols and saponins in E. elephantina root extracts. Quantitative phytochemical analysis of alkaloids in water extract was 932.44 ± 1.23 mg. Condensed tannins, phenols and saponins in ethanol extract were 450.52 ± 4.56 mg CE/g, 476.11 ± 0.03 mg GAE/g and 832.85 ± 1.45 mg respectively. Flavonoids was 803.93 ± 0.02 mg QE/g in methanol extract as the highest recordings. In vitro screening of ethanol, methanol and water extracts of E. elephantina for potential anthelminthic activity against adult Paramphistomum cervi worm motility showed time and dose-dependent significant effects after 12 hours of treatment. Ethanolic extracts showed a highly significant (P < 0.05) inhibition of motility (86.67 and 96.67 percent) at concentrations 7.5 and 15 mg/ml, respectively. Methanolic and aqueous extracts exhibited a significant (P < 0.05) motility inhibition (96.67 and 66.67 percent, respectively) at concentration of 15 mg/ml 16 hours post-exposure. In vitro assessment of structural changes to adult gastro-intestinal worms following exposure to E. elephantina extracts was done using scanning electron microscopy (SEM). The SEM observations revealed structural changes in the worms after contact with E. elephantina extracts in comparison with the control worms kept in Phosphate-buffered saline (PBS). The major alterations were around the cuticle, posterior and anterior areas. These changes included longitudinal and transversal folds and thicker cuticula ridges, material aggregates around the posterior and anterior areas. The study showed that ethanol, methanol and water extracts of E. elephantina root contained significant amounts of alkaloids, condensed tannins, flavanoids, phenols and saponins. Validation studies showed that extracts of E. elephantina possess anthelminthic activities. The structural changes found in the worms exposed to extracts of E. elephantina root extracts affected their motility and nutrition, resulting in mortality.
- Format
- 143 leaves
- Format
- Publisher
- University of Fort Hare
- Publisher
- Faculty of Science and Agriculture
- Language
- English
- Rights
- University of Fort Hare
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UFH Department of Livestock & Pasture Science
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | MSc (Animal Science) MAZHANGARA, IR.pdf | 2 MB | Adobe Acrobat PDF | View Details Download |